Size | 1000 ml |
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Indicator to presumptively identify indoles and urobilinogen
Application: Erlich reactive is alternative to Kovac’s reagent for idols, but the test is more sensitive. It needs organic extraction with ether or xylene before use. As far back as 1889, the indole test was used as a means to distinguish between Escherichia coli and Enterobacter aerogenes .The numerous variations of the indole test alone and in combination with other biochemical tests attest to the central role this test has played in the characterization of coliforms (gram-negative nonsporulating bacilli that ferment lactose, producing acid and gas). The indole test is still used as a classic test to distinguish indole-positive E. coli from indole-negative Enterobacter and Klebsiella. The indole test screens for the ability of an organism to degrade the amino acid tryptophan and produce indole.
N.B!: The clinical interpretation of any staining, or its absence, should be complemented by morphological studies and proper controls, and should be evaluated within the context of the patient’s clinical history and other diagnostic tests by a qualified pathologist.