LEE stain

Additional information


250 ml

This stain is often used for general purpose tissue staining.

Application: Detection of early stages of ischemia changes of myocardium. The first six hours postinfarction are the most difficult to diagnose due to the lack of visi- ble morphological change or reliable histochemistry. The HBFP stain introduces by Lie (Lie et al. 1971) has been reported to give a positive staining reaction 30 min after coronary occlusion and remain positive up to 36 h. This technique has hitherto been the only reasonably reliable method of demonstrating early ischaemic changes. False positive or false negative staining could be due to aging of the unstained sections in room air, differentiation problem (over-/under- differentiated sections), aging of solutions.

N.B!: The clinical interpretation of any staining, or its absence, should be complemented by morphological studies and proper controls, and should be evaluated within the context of the patient’s clinical history and other diagnostic tests by a qualified pathologist. All reagents have been optimally prepared for use and require no mixing or diluting.